Sentence examples for solution and fixation from inspiring English sources

Briefly, cells were arrested at metaphase by adding 100 ng/ml of colcemid (Sigma) to the culture media, followed by incubation with pre-warmed hypotonic solution and fixation (methanol:acetic acid in 3 : 1).

Infiltration with GUS substrate solution and tissue fixation followed Millet et al. (2010).

Internalization of the antibody-bound FPR was stopped by washing with cold modified Hanks balanced salt solution and by fixation with 3% paraformaldehyde and 2% sucrose.

After washing with the buffer solution and post-fixation in 1% OsO4 and 0.1 m cacodylate buffer (pH 7.4), they were washed with the buffer solution, dehydrated using alcohol and acetone, and embedded in epoxy resin.

In-house solutions for lysis and fixation were used.

It enhanced P availability by 68%% over the control in soil solution and reduced P fixation capacity from 43 % (control) to 32%% by addition of bagasse and press mud (5 g kg−1 of soil) (Fig. 2).

Shortly, the samples were processed through disaggregating by homogenizer, treatment with acetic acid solution (45 60% w/v), and fixation with methanol/acetic acid mixture (3:1).

All media and fixation solution were aspirated, and cells were washed once with 200 µL warm PBS++.

Cells then were re-suspended and incubated for 20 min in 2 ml lysing solution for lysis of erythrocytes and fixation of the leucocytes.

Prepare fresh 4% formaldehyde solution for tissue specimen dissection and fixation (refer to Table A1 for reagent and equipment details): a.

Fix tissues overnight at 4°C. 1. Prepare fresh 4% formaldehyde solution for tissue specimen dissection and fixation (refer to Table A1 for reagent and equipment details): a.