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Each pot had 2.5 L of aerated nutrient solution and contained four plants.
Copper was electrodeposited from a laboratory-made solution and contained 187.5 g/L of sulfate pentahydrate.
This was the stock solution and contained approximately 6.4 g of metal in 1 ml of PBS.
The medium used for all experiments was prepared from a 10× stock solution and contained (final concentration in g L 1) NH4Cl3 2.5, NaH2PO4.5, NandPO4 0.6, and KCl 0.1.
The extracellular recording solution was the artificial cerebrospinal fluid (aCSF) solution and contained the following (in mM): NaCl, 125; KCl, 2.5; D-Glucose, 25; CaCl2, 2; MgCl2, 1; NaH2PO4, 1.25; and NaHCO3, 25 (320 mOsm).
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A solution containing CTAD (1 mM) was titrated into a solution containing FIH (1.5 μM) while the fluorescence at 330 nm was monitored (λex = 295 nm); both solutions were anaerobic and contained CoSO4 (25 μM) and αKG (500 μM).
The discs were soaked with double distilled water, plant leaf extracts, silver nitrate solution and solution containing silver nanoparticles of each type separately.
Three different PN solutions were used: a soya oil containing solution [long chain fatty acids LCT ], an olive oil containing solution, and a solution containing middle and long chain fatty acids MCT/LCT).
Wetting solution contained 100%% acetonitrile, equilibration solution and wash solution contained 0.1 % TFA in water, and elution solution contained 0.1 % TFA in acetonitrile water (50 50).
For children ages 6 8 years, math problems and solutions contained only single-digit numbers.
Pipette and bath solution contained 200 mM KCl, 40 mM MgCl2, and 5 mM HEPES (pH 7.2 adjusted with KOH).
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