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Intake of protein, lipid, carbohydrate and energy for each day will be calculated by multiplying intake volumes for each solution administered by the nutrient concentration obtained from manufacturers specifications or, for breast milk, published data [ 119].
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Racemic ketamine (2 mg/mL solutions) or saline solution were administered by a computerized target-controlled infusion (TCI) system, which calculates the infusion rates required to achieve the "target" blood concentration set by the user.
General anesthesia was done by intraperitoneal injection of a nonbarbiturate anesthetic in physiological solution administered according to the supplier recommendations (sodium-thiopental solution (Farmotal, Pharmacia & Upjohn, USA)).
The therapy solution was administered by slow intravenous injection over 1 min followed by 1000 ml of NaCl or Ringer.
For in vivo studies, both MY-24 and ribavirin were dissolved in sterile saline solution and administered by intraperitoneal (i.p).
The solution was administered by a custom made nebulizer flowing at 4 L/min of O2 for 25 min into an airtight chamber containing 5 7 mice.
Glucose (1 g/kg body weight; 20% glucose solution) was administered by intraperitoneal injection.
The GEM solution was administered by intravenous drip infusion over 30 min.
The therapy solution was administered by intravenous infusion over 20 min.
γ-Secretase inhibitor XX and saline solution were administered by intraperitoneal injection.
Docetaxel was reconstituted in 250 ml of 5% glucose solution and administered by intravenous infusion over 60 min.
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