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After incubation for 4 h, the formazan crystals were solubilized with 150 μL of DMSO.
The ethanol extracts were combined, concentrated to dryness and solubilized with one mL of distilled water.
Subsequently, the solution was aspirated and the insoluble formazan product was solubilized with acidified iso-propanol.
OmpC from IBs was solubilized with 4 M urea and refolded.
Hemicellulose and lignin are solubilized with minimal degradation, and the hemicellulose is converted to sugars with acid pretreatment.
The plates were air dried; the bound dye was solubilized with 200 μL of 33% (v/v) glacial acetic acid.
The dye incorporated by the adherent cells was solubilized with 200 μl of 95% (v/v) ethanol.
Adhered safranin was solubilized with dimethyl sulfoxide (DMSO) and absorbance of the wells were measured at 490 nm wavelength.
Fig. 2 Optical characteristics of the CdSe/ZnS core/shell quantum dots solubilized with HS− PEG 12−COOH ligands.
The suspension was solubilized with an equal volume of resuspension buffer containing 2%% (w/v) dodecyl-β-D-maltoside.
After aspirating the culture medium, the formazan crystals formed were solubilized with 200 μL of organic solvent.
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