Sentence examples for solubilized using from inspiring English sources

Assay was stopped by removing the medium on ice and membranes were solubilized using KIRA-ELISA solubilization buffer (NaCl 150 mM, Hepes 50 mM, Triton X-100 0.5%, thimerosal 0.01%, sodium orthovanadate 2 mM, supplemented with a cocktail of protease inhibitors) for 1 h at room temperature.

Nystatin was solubilized using Tween 60 and was incorporated into the selected film.

This ensemble of the metal decorated hybrid structure was solubilized using Nafion and a simple drop casting method was employed to fabricate the sensor.

Unpurified, non-citraconylated starting inclusion body protein (i.e., crude inclusion body protein) was solubilized using SDS detergent and run on SDS-PAGE gels for direct comparison to the purified C-rmCRP protein.

The protein aggregates was solubilized using high concentration of Urea while β-Mercaptoethanol was added to split the disulfide bond (that are responsible of the inter-subunit aggregation) and to maintain cysteine residues in a reduced state.

Formazan crystals were solubilized using 1∶1 ethanol:DMSO solution.

Approximate half of the cys-mutated rmCRP in the starting inclusion body sample was found to directly solubilize using the method described here, and half required a brief exposure to high pHs to achieve solubilization.

The protein was expressed in E. coli (M15 strain) (Qiagen) that were lysed by sonication and purified from DDM solubilized membranes using TALON Metal affinity chromatography (Clontech Laboratories, Inc, Mountain View, CA), followed by a further purification step using fast protein liquid chromatography (FPLC; Superdex 200 10/300 GL column, GE Healthcare, Pittsburgh, PA).

The protein was solubilized, purified using nickel affinity chromatography, and then cleaved with 0.5 mM NiSO4 at pH 9.0 and 45 °C, all in 6 M guanidine-HCl.

The expressed rMEHB formed inclusion bodies that were disrupt and solubilized by using 6 M urea.

However, all tissues were solubilized sufficiently using the 2 M MgCl2 + 1% Lutensol AO-07 lysis buffer (Table  1).