Sentence examples for solubilising reagent from inspiring English sources
Afterwards, 100 μl of solubilising reagent was added and the plates were incubated at 37°C overnight.
Inclusion bodies were retrieved from cell pellets and solubilised using BugBuster™ reagent with 0.3 μl ml−1 Benzonase and Novagen Refolding Kit (both: Novagen, Madison, WI, USA) according to the manufacturer's instructions.
Cells were incubated with MTT reagent (5 mg/ml) for 4 hr, solubilised in dimethyl sulfoxide, and the absorbance at 570 nm was measured.
The pellets obtained were solubilised by incubating at 100°C for 5 minutes in Reagent C. The method for the ELISA was carried out as described by the manufacturers.
The reagent was then removed and the crystals were solubilised using isopropyl alcohol (IPA).
After labelling with a fluorescent lysine-reactive reagent, Cy5-NHS ester, A- and D-forms were solubilised and applied on a 4 13% BN-PAGE (Fig. 1A).
There is barely enough reagent to test blood donations.
We had need for a specialised reagent: oligonucleotides.
I could not even pick up reagent bottles freely.
I was the limiting reagent.
