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The solids were transferred to 1 L centrifuge jars, centrifuged at 1105×g for 5 min and the cake stored at 4 °C.
The resultant solids were transferred to alumina crucibles, heated at 5 °C min−1 to 600 °C and kept at this temperature for 10 h.
The pelleted solids were transferred to fresh containers for freeze-drying, and the aqueous fractions were centrifuged repeatedly at 13 500 × g for 15 min intervals at 4 °C until no further pellet was observed.
25 3 Next, the amorphous desolvated solids were transferred to a vial and exposed over 72 h to the vapours of H2O, a 50 50 % (v %) mixture of H2O and EtOH, EtOH and MeOH (c, d, e and f in Figure 3).
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The brines, which then contain 19 21 percent sodium chloride and 28 30 percent total dissolved solids, are transferred to another pond to crystallize the salt.
To begin sample preparation, a sample (aqueous or solid) is transferred into the distillation flask and dried.
A weighed amount of the dried solid was transferred into a flask and soaked in a certain amount of mixed acids of H2SO4/HNO3 (3:1 volume ratio) and sonicated at 60 °C for 12 h.
The solid was transferred back into a clean flask containing a mixture of EDA (4 mL: 73.84 mmol), dissolved in dried tetrahydrofuran (300 mL) and refluxed for 24 h.
The solid samples were transferred to a carbon tape held by an SEM sample holder for analyses.
(3) Final phantom preparation: The GNR phantoms (already in the solid state) were transferred to cell culture plates (∼1.55 cm diameter), and the solutions of the fluorescent basis phantoms were added.
One-week-old seedlings grown on half strength MS solid media were transferred to filter paper soaked with 0.05% DMSO, 50 μM corresponding compound or -ABA for another 1.5 h and luciferase imaging was carried out as described before.
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