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Transgenic (TG) and wild-type (WT) control switchgrass lines were DA pretreated and washed solids were subjected to SSF.
In the second configuration, the slurry was pressed and washed, and the solids were subjected to SSF.
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Solids are subjected to hydrolysis (also called second stage hydrolysis).
When bulk particulate solids are subjected to an electric field, a remarkable phenomenon occurs as manifested, e.g. in the electro rheological fluids and in a device developed for controlling the flow of granular materials, the electromechanical valve for solids (EVS).
In the process shown in Fig. 3d, the solids in the acid hydrolysate are washed to remove residual soluble sugars, and the solids are subjected to a pretreatment step.
In longitudinal waves (also called compression), dilatational, or irrotational waves, the solid is subjected to alternate local compressions and expansions.
The residual solid was subjected to enzymatic hydrolysis, and the highest sugar concentration of 30.5 g/L was obtained.
The solid was subjected to flash column chromatography (silica gel; benzene followed by benzene/acetone (10 1)) to give crude 12.
The remaining solid was subjected to flash chromatography on silica gel (toluene/EtOAc, 3 1) to afford 3 (17.21 g, 61 %) as a colorless solid.
The experiment was stopped, the solvent was removed under reduced pressure, and a sample of the remaining solid was subjected to NMR analysis to determine the conversion (80%%).
While adding the oxygenated distilled water, the semi-solid was subjected to vigorous stirring to form an emulsion, of which 200 μl will then be added to each well that has been preloaded with 10 μl of the samples or the positive control (BHA).
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