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The proposed improvements from this study have been validated for both low solids conditions and at endpoint conversion conditions for high solids.
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Furthermore, as both overexpressed genes contain CBM family 2 domains, this CBM may be effective at binding cellulose under thermophilic, high-solids conditions and may be useful for engineering other cellulolytic enzymes tolerant to these conditions.
Power consumption required to suspend water solid slurries in a mechanically agitated tank has been studied over a wide range of design and solids conditions with the goal to improve the agitation energy efficiency.
The enzymatic cellulose digestibility of deacetylated corn stover (DCS) treated by the disk refiner was measured at high solids conditions (15 wt% insoluble solids).
Similitude method is applied to establish the dynamic similarity among LSCFBs by tuning physical properties of liquids and solids, operating conditions and bed dimensions to match several scaling sets of dimensionless groups.
As a proof of principle system, we chose a small, well-folded 15 nt hairpin RNA, 3. The RNA was assembled under typical RNA solid phase conditions and the TEMPO amidite was attached at the 5′-terminus using standard conditions with the activator 5-benzylthio-1 H-tetrazole and 2 min coupling time.
Expression vectors with IRES-dependent GFP-expression were used to monitor the proportion of GFP-positive cells in competitive proliferation assays performed in liquid and semi-solid conditions.
Despite shifts in the community with storage, the samples were active upon revival under thermophilic and high-solid conditions.
Their unique presence and high expression level in the thermophilic community make them promising candidates for improving deconstruction processes under high-temperature and high-solids conditions.
Experiments were done to determine the number of enrichment passages necessary to achieve a stable microbial community capable of degrading switchgrass under thermophilic and high-solid conditions.
The results support our hypothesis that this reactor system will work well for a variety of biomass types and pretreatment severities at high-solids conditions.
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