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The tube was allowed to cool and the solids collected by filtration.
In brief, a 1 ml sample of the culture broth was withdrawn and the solids collected by centrifugation.
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In contrast, the solid collected by destabilization of the colloid solutions is composed of agglomerated nanoparticles (Figure 3). Figure 3a,b shows field emission gun-SEM images of the as-obtained solid.
The concentrated filtrate was sonicated in hexanes (20 mL/mmol) and the solid collected by filtration to give the product, which was purified by recrystallization from acetone.
The white solid collected by freeze-drying was further extracted with cold methanol for 24 h to remove rests of the PNIPAAM homopolymer.
At the end of the experiment, the solids were collected by centrifugation (10,000 rpm/min) and filtration and then were washed thoroughly by using ethanol and deionized water.
The reaction mixture was heated to reflux for five hours, cooled and the precipitated solids were collected by filtration to give the ketones 3a-d which were pure enough to be used in the next step.
Then buserite solids were collected by centrifugation and re-suspended in 400 mL 1 mol/L MgCl2 solution and transferred to a 1 L triangular flask connected with a condensation device, then heated to reflux at 100°C and maintained at reflux conditions under magnetic stirring.
The solids were collected by filtration and then washed with the methanol:water mixture (1 × 50 mL), followed by water (2 × 50 mL).
The suspended solid was collected by filtration and washed with water to afford the key intermediates 3 as pale yellow solids, which can be used directly for the next reaction without further purification.
The resulted solid was collected by magnetic field and washed with ethanol and dried at 60 °C.
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