Four ml of agar/medium was placed into 60 mm tissue culture dishes and allowed to solidify (base agar).
Briefly, 1.5 ml FBS supplemented medium containing 0.5% agarose were added in 35-mm cell culture dishes and allowed to solidify (base agar).
For the soft agar assay, a 2-ml mixture of complete DMEM and 0.7% low-melting-temperature agarose (Seaplaque: Lonza, Rockland, ME, USA) was added to a six-well tissue culture dish and then allowed to solidify (base agar).
The partnership has also been solidified based on a recent decision by Microsoft's global office in Seattle, USA, to make South Africa one of its five priority countries for investment in education.
"In my 15 years in Yonkers, I've never seen the N.A.A.C.P. more involved in recruiting and solidifying its base.
Right now, he's trying to square the circle, solidifying the base while asking the Wall Streeters for cash.
"The old strategy of just going to certain states and solidifying the base — I don't think that works anymore," he said, adding, "Not only that, but I think it would be boring".
Although the unrelenting barrages have enabled the candidates to solidify their bases -- a distinct advantage for Mr. Allard in a state where Republicans outnumber Democrats by 185,000 of the 2.8 million registered voters -- polls show that 310,000 voters are undecided.
Historically this kind of scapegoating is used by politicians to solidify their bases and draw attention away from their failing policies, and no doubt this is what's happening in Russia.
Which is why it once seemed safe to assume that Obama's social issues strategy was a way of solidifying his base, and a warm-up act for the fall campaign.
