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For all ant colonies, the fungal cultivar was isolated by applying garden substrate directly onto Potato Dextrose Agar (PDA) (39 g/l; Becton, Dickinson & Co., MD 21151, USA); supplemented with 5 g/l of agar (Becton, Dickinson & Co., MD 21151, USA), added as a further solidifying agent, and Streptomycin sulphate and Penicillin-G (both at 12.5 mg/l), included to inhibit bacterial growth.
For solid cultures, 1.5% gellan gum (Sigma) was used as a solidifying agent.
Another important factor to explain this diversity is the use of gellan gum instead of agar as a solidifying agent.
Agar is widely employed in biological laboratories as a solidifying agent for growth media for microorganisms and in the bakery industry as a gelling agent; it forms a part of the diet of people in several areas of East Asia.
Two-week-old friable calli were used to initiate cell suspension culture in the above mentioned MS medium without agar (solidifying agent).
The composition of skim milk agar medium was (g/l): casein 5.0 g, glucose 1.0 g, skim milk powder 3.0 g, yeast extract 2.5 g and agar 15.0 g as solidifying agent.
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Aminopropyltriethoxysilane (KH-550), a silane coupling agent that can crosslink with resin and iron base material, was used as curing agent for solidifying this GMA modified resin.
As early as December 2001, intelligence officials detected evidence that Al Qaeda was reinventing itself, solidifying alliances with other terror groups and permitting midlevel agents to plan and execute attacks.
The core of this roster, including Ovechkin, Holtby and playmaker Nicklas Backstrom, was in place when the pair were hired but the peripheral moves, including a trade for forward TJ Oshie and a move for former Conn Smythe Trophy winner and three-time Stanley Cup champion Justin Williams as a free agent have solidified their place as one of the few legitimate Stanley Cup threats.
Based on the concept of stoichiometric mass fractions for resin, curing agent and solidified material the bulk compression modulus as well as the bulk heat- and shrinking dilatation coefficients are derived and compared with ad hoc assumptions from the literature.
The body was cooled with ice to solidify the injected contrast agent.
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