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The remaining liquid will solidify under near-equilibrium conditions as the latent heat is removed.
Sterilized 20 ml PDA with the additives of the concentrations (0.1, 0.5, and 1.0%) were incorporated into 25-mm diameter sterile petri dishes, and they were allowed to solidify under laminar flow cabinet.
The cell/agarose suspension was used to form the second layer and allowed to solidify under a fresh coverslip at room temperature for 5 min.
A volume of 75 μl of this second layer was quickly pipetted on to the NMP agarose-coated slides and allowed to solidify under a coverslip for 5 min on ice.
The pellets were resuspended in 80 μl of 0.6% low melting point agarose (dissolved in Ca -and Mg-free PBS at 37°C), layered onto the normal melting point agarose and allowed to solidify under a fresh coverslip on ice.
A measure of 80 μl of cell/agarose suspension (10 μl of cell suspension containing about 10 cells was mixed with 70 μl of 0.6% low-melting-point agarose, Roth, 6351.1, in PBS) was placed over the first agarose layer and allowed to solidify under a coverglass.
Similar(52)
Racially discriminatory practices, in place from the formation of the country and expanded over the decades, are further broadened and solidified under the party's policy of apartheid, which sanctions racial segregation and political and economic discrimination against nonwhites.
The Energy Department itself has announced many cleanup timetables over the years but had never made clear how much of the waste it would proceed with solidifying under its plan to exempt some of it.
Unlike all other isotopes of the elements in the periodic table, neither 3He nor 4He solidifies under low pressures at a temperature near absolute zero, 0 Kelvin (K) (−273 °C, or −459 °F).
For months, Bridget took care of him and their family, and, when he was better, their marriage was better, too; it had solidified under the stress, like a building settling on its foundation.
It had universal appeal: brutal in fact, but seemingly idealistic, the system solidified under Stalin won admiration in many poor countries, and from a host of clever fools in rich ones.Hitler's notions were woollier, more mystical, resting on such concepts as will, authority, racial superiority and worship of the leader.
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