More stringent and appropriate controls, and more experimental settings, may help to solidify their conclusions.
However, many of the interpretations rest on structural modeling and some initial lipidomics in cell culture, and there are several experiments that would solidify their conclusions.
You reference a chloride ion transport study in your paper and perhaps the approach taken previously could be implemented to solidify your conclusions about the clcA mutants.
Prospective research to verify these results in an array of ED practice settings (e.g., non-cancer centers, community EDs) is needed, however, to solidify this conclusion.
These findings seem to solidify the conclusion that contraction does not occur by caspase 3 dependent apoptotic cell death.
Taken together, these data solidify the conclusion that in association with Ago2, diRNAs play a specific role in HR repair.
JCK coordinated the study, narrowed the initial study focus, participated in the design of the study, developed the methodology directed the research project, vetted the manuscript, refined the analysis/discussion of results and solidified the conclusions.
The use of AICAR is equivocal, as other effects of AICAR have been shown to be mediated by pathways independent of AMPK and it is not a fully definitive reagent to use to solidify a conclusion about AMPK.
Most important in terms of solidifying the conclusions, however, are Reviewer 1's and 3's similar points about the demonstration of the qualitative reason for the improvement of the current model (Reviewer 1) and the absence of a reasonable analysis of other (sub-optimal) models (Reviewer 3).
Nonetheless, further trials may need to be performed to solidify such a conclusion.
For these reasons the reviewers request direct fractionation experiments (analyzing Rab3 levels in the membrane and soluble fractions) to solidify this key conclusion.
