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"The idea was to create an absolute solid whole," Mr. Moeller said, explaining that the symbolism held religious significance.
So the aggregate of atoms is not a solid whole but rather there is space among atoms.
Solid whole transcriptome libraries were made as outlined in the Solid Whole transcriptome kit protocol (Applied Biosystems, Cat. No. 4425680).
Whole transcriptome reads were aligned using AB's SOLiD Whole Transcriptome Pipeline [72].
SOLiD whole transcriptome libraries were made according to the SOLiD Whole transcriptome kit protocol (Applied Biosystems) and the library concentrations were measured with the Quant-it HS dsDNA assay kit (Invitrogen).
RNA-Seq: a sequencing library was created from 1.6 μg of mRNA using the SOLiD Whole Transcriptome Analysis Kit.
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High-throughput sequencing libraries were prepared according to the SOLiD whole-transcriptome library preparation protocol (Ashton-Beaucage et al. 2010).
Although the length of each remaining read sequence is 50 nt, four nucleotides from the 3′-terminus were excluded for accuracy, following the SOLiD whole-transcriptome analysis protocol.
There are several reasons for selecting high solids, whole slurry enzymatic hydrolysis.
High solids enzymatic hydrolysis using unwashed, pretreated solids was performed in order to determine 1) the deacetylation effect of high solids, whole slurry enzymatic hydrolysis; and 2) the toxicity of acetate in enzymatic hydrolyzates.
To prepare for experiments using washed solids, whole slurry PCS was washed using a basket centrifuge (Model STM-2000, WeStatesStates, Hamilton, OH, USA) with a nylon filter bag (105 μm pore size, 25% open area, Sefar filtration cloth, Western States) until the glucose concentration in the wash water was below 0.1 g/l.
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