Although Pico Profiling also suffered from this compression of dynamic range, it still provides far more true positive measurements than SOLiD sequencing of picograms of RNA (60% versus 43% respectively).
Microbiota gene content was determined by high-throughput SOLiD sequencing of total faecal DNA [ 25].
SOLiD sequencing of ChIP-Seq libraries were performed on an ABI SOLiD4 instrument with 35-bp reads according to manufacturer's instructions (Life Technologies, Grand Island, NY) by the UTSW McDermott DNA Sequencing Core.
SOLiD sequencing of four V. vulnificus strains representing different clades (1 and 2) and biotypes (1 and 2) was used for comparative genomic analysis.
SOLiD sequencing of multiple bacterial genomes of V. vulnificus and subsequent comparative genomic analysis identified numerous genes that are common to the most virulent strains yet lacking from attenuated strains for which genomic DNA sequence data are available.
Twice as many SNPs were identified using SOLiD sequencing of genomic DNA than cDNA, which is not surprising since SNP discovery in cDNA is limited by the number of genes sampled by the cDNA library.
We performed SOLiD sequencing on the genomes of four V. vulnificus strains to increase the understanding of the genetic differences between the two major clades and the biotypes of this organism and to possibly identify sequences associated with differences in virulence potential in our subcutaneously inoculated iron dextran-treated mouse model [ 23- 25].
In the present study we used the Applied Biosystems SOLiD sequencer for sequencing the metagenome of a human fecal sample by 16S rRNA sequencing and also by shotgun sequencing of mate-pair libraries to investigate the suitability of SOLiD sequencing for metagenome sequencing.
For SOLiD-sequencing of 50 bp single-end reads, single fragment libraries were generated from 5 μg sheared DNA with the fragment library core kit.
