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A solid sandwich: not offensive, but not brilliant.
The meat was clearly good quality, as was the bread, and the little jug of gravy to pour over your skinny chips was a nice touch, but where was the extra crackling, apple sauce or stuffing to turn this solid sandwich into a gourmet treat?
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A solid phase sandwich enzyme-linked immunosorbent assay (ELISA, Cell Signaling) was used to detect and quantify the cleaved Csp-3, according to the manufacturer's instructions.
To determine levels of MCP-1, IL-6 and TGF-beta in cell culture supernatants, commercial solid phase sandwich ELISA kits (BD OptEIA™, BD Biosciences, Heidelberg, Germany) were used according to the manufacturer's instructions.
IL-8, IL-10 and TNF-α were measured in cell tissue culture supernatants with solid phase sandwich ELISA kits (BioSource International, Camarillo, CA) with the following lower limits of detection: 0.39, 7.8, and 1 pg/ml, respectively.
A solid phase sandwich ELISA was used to measure plasma concentrations of VEGF.
This assay is a solid phase sandwich ELISA using 2 kinds of highly specific antibodies.
It is a solid phase sandwich enzyme linked immunosorbent assay (ELISA) using a microtitre plate reader at 450 nm.
Serum amyloid A (SAA) concentration was determined with a commercial solid phase sandwich ELISA (Phase SAA assay, Tridelta Development Ltd, Ireland).
In order to determine the levels of TNF-α and IL-1β, a commercial solid phase sandwich ELISA from Biosource International (Camarillo, CA, USA) was used.
Briefly, the assay was a solid phase sandwich ELISA in which a monoclonal antibody, specific to the cytokine of interest, was coated on a 96-well plate.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com