The process was reminiscent of Pollock's own drip method, which the contemporary artist emulated but also personalized by watering down her paint so that they appeared as washed out spreads more than solid overlays.
If that be taking solids and overlaying with prints.
At first, HUH-wt, HUH-PAS, and HUH-REISO cells were pre-cultured under conventional conditions or under a thin layer of solid medium ("agarose overlay", Figure 9D-F) for six weeks.
Protonemal tissues of Physcomitrella patens subsp. patens were subcultured at weekly intervals on the surface of cellophane that overlayed solid BCD medium containing 5 mM ammonium tartrate and 1% glucose (BCDAG) [ 63].
To test this, the liquid medium overlaying the solid minimal medium (Figure 1A) was replaced by deionized water.
Liquid one-step growth curves, agar overlays and solid plate stocks were performed as described previously [1], with the addition of the appropriate antibiotic types/forms (disks or solutions) to the appropriate media.
The plates were incubated at room temperature until the overlay media became solid, and then they were placed in a 5% CO2 humidified atmosphere at 37°C.
Histograms show CD44, CD5, CD69 or TCRb expression on gated DP, CD4SP or CD8SP thymocytes from WT (solid gray) vs chino (red overlay) mice, representative of four separate experiments (n = 7 mice per group in total).
Priester et al. [ 9] demonstrated that unsaturated biofilms of P. putida strain mt-2 on membranes overlaying iron-deficient solid media either containing dichromate or solid dichromate-coated hematite were able to completely reduce Cr VI) under both conditions.
The shape-VV task employed a second visual stimulus, which was a novel solid red shape constructed by overlaying rectangular, triangular and circular shapes in different combinations (see Figure 1, shape-V).
And we always acknowledge that clients and potential clients know their businesses best; our role is to overlay that knowledge with solid marketing advice and the ability to help focus messaging to the target audience.
