The purified beads were then loaded onto a SOLiD 6-lane Flowchip and incubated upside down for 1 hour at 37°C.
Faced with those narrow lanes, usually solid with tourists, and, at night and weekends, mobs blocking the sidewalks outside gay hook-up spots such as L'Open or Cox, I detour.
Addition of glucosamine-6-phosphate (GlcN6P) induces site-specific self-cleavage of the glmS ribozyme immediately 3' to the target RNA (Figure S1), releasing the co-transcriptionally folded target RNA with homogeneous 3' end from the solid support (Figure 1b, lanes G1 G2).
A magnetic particle concentrator (MPC) was applied to remove the supernatant and yield a solid bead pellet (analyzed in lane "T" in Figure 1b).
If you follow the rules of the road, and you wait to merge in until the broken line starts, you will often have honking jerks on your tail who have ignored the double solid lines and entered the lane before it really begins!
Both lanes are blocked solid, and the offender, usually in an S.U.V., pulls onto the edge of the road, often with one or both tires on the shoulder, and charges forward until there's no more space, at which point he forces his way in.
The Bedford Riding Lanes fall pace was booked solid three days before the September event, Mrs. Bancel said.
THE Park Avenue automobile tunnel under Murray Hill used to be pretty hairy, a 1,500-foot-long 1,500-foot-long 1,500-foot-longlid walls on each side, not much clearance and cars barreling along at highway speeds.
After collecting the target RNA within an hour of completion of the transcription reaction, the strong biotin-streptavidin interaction can be broken [32] to recover the solid support for further use (Figure 1b, lanes R1 R4).
Let's just take a quick trip down memory lane out of respect for a solid phone.
