We detected solid expression of HCC in the epithelium of recurrent pterygium (key in Figure 4C) in cells from the basal, middle and apical layers, and we could quantify by morphometry.
In neonatal mice, transgene expression was found to be already increased in ventricular CMs compared to late embryonic hearts (P0 in Fig. 4a) and on P3 solid expression of the H2B-mCh protein was detectable in ventricular and atrial CMs (P3 in Fig. 4a), as demonstrated by high overlap of α-actinin staining and H2B-mCh expression after dissociation (Fig. 4b).
For comparison of the four microarray datasets downloaded from GEO with the SOLiD gene expressions profiles, the microarray gene expression levels were averaged across the probes and assigned HGNC gene names using GEOquery R package.
For comparison of the Illumina datasets with the SOLiD gene expressions profiles, the EnsEMBL gene names available for the ENCODE GENCODEE annotation) and van Delft et al. (EnsEMBL v58 genome) data were converted to HGNC gene names using biomaRt R package.
To prove the authenticity of the SOLiD, the expression levels of selected genes were validated on cultured cell lines from other cell lines of posterior UM and NUM by qRT-PCR.
In solid tumours, expression levels of MBD2 (57/71) and MeCP2 (64/71) were significantly reduced in the majority of primary tumours as detected by quantitative real-time RT-PCR.
CD24 expression is abundantly expressed in human solid tumors; its expression is associated with high tumorigenic capacity and worse prognosis [ 35].
Most of the studies focusing on TKTL1 expression in solid malignancies examined TKTL1 expression levels via immunohistochemistry, whereas expression on cDNA level has been examined less frequently.
Since the methods used reflect process relevant conditions, they will help to quickly translate screening success to industrial success, without the potential pitfalls of changing substrate, solids loading, expression host, or protein background.
Colony-forming assay on the cellular sensitivity to cisplatin showed that the three cell lines with low MAD2 protein expression (dotted lines) were more resistant than the ones with relatively high MAD2 expression (solid lines).
