However, the heterogeneous characteristics of cells grown on solid media introduce difficulty when testing for functional genes.
Existing protein immobilization approaches, including noncovalent or covalent attachments to solid supports, have difficulties in controlling protein orientation, reducing nonspecific absorption and preventing protein denaturation.
Therefore, in the author's opinion, the presence of ARSA only justifies surgical intervention in those patients in whom the classical pattern of solid feeding passage difficulties "dysphagia lusoria" is present.
But even that support has limits, which goes far in explaining why American leaders are having such difficulty getting solid Arab backing for military action in Afghanistan.
And while all the performances are solid, I often had difficulty in believing these characters as something other than figures in a parable.
But the solid evidence of the difficulty of Turner's art lies within the work itself, a vast body of oil paintings, watercolors, drawings and prints that he began producing as a child prodigy and continued to make, without letup, almost until the end of his life.
It revealed that she undertook adeno-tonsillectomy under local anesthesia at the age of 23 in China, and since then she had been suffering from dysphagia (difficulty swallowing solid food).
During follow-up, the majority of patients were able to eat a normal diet or ate solid food with some difficulty (dysphagia score 0 1).
The screening assays were performed using solid media due to difficulty in the determination of lipolytic activity as the water soluble lipases catalyse reaction of only the water insoluble substrates [ 1, 21, 22].
Dysphagia was graded according to a published method (0 = no dysphagia, 1 = difficulty swallowing solid food, 2 = able to swallow soft food, 3 = able to swallow liquids, and 4 = unable to swallow food).
