In one of these six cases we know that the intronic deletion and the other point mutation (an exon 2 splice donor site mutation, c.164+1 G>A) are in trans as PCR amplification of exon 2 using a forward primer from within the intron 1 deletion solely amplified the allele containing the splice site mutation.
It only amplified her voice.
Cancer vaccines are solely meant to amplify the pool of type 1 cytokine oriented CD4+ and CD8+ T cells that recognize tumor antigen and ultimately foster control and destruction of a growing tumor.
Moreover, the applied primers are known to amplify solely the mitochondrial copy of the gene in hylobatids [ 34], and for cross-validation purposes, for some specimens, sequences were generated using different material types (blood, faeces).
A PCR experiment using primers with opposite orientations designed to amplify solely TIR-220 repeats resulted in a "ladder-like" pattern of amplicons representing multimers (up to seven) of approximately 220 bp, confirming the tandem organization of TIR-220 repeats seen in the assembled genome of D. virilis (supplementary fig. S3, Supplementary Material online).
Each of ~15 ng tracerA1 and tracerA2 were hybridized with ~1,500 ng driver for 20 h at 60°C. 2 μl of the hybridization result was used as template to amplify solely tracer fragments using the adapter primers A1 (5'-TGTAGCGTGAAGACGACAGAA-3') and A2 (5'-TGTAGCGTGAAGACCTGTCTT-3').
To further investigate the application of the UCD COS, we evaluated the ability of 96 primers designed solely for tomato to amplify single-copy sequences in pepper and eggplant.
The similar reasoning of the high fidelity viral RNA polymerases envisioned above for IAV Pol can be applied to polioviruses which also amplify their RNA genomes multiple times per infection solely by their own high fidelity RNA polymerase.
In communities with a high rate of primary drug resistance, approaches which assign treatment regimens based solely on the patient's history of prior treatment may amplify drug resistance [22].
With appreciative inquiry, the researcher was also able to explore and amplify what works and the successful elements and experiences that could inform learning and change without solely focusing on problems.
These competitive dynamics are driven solely by changes in financial incentives and are not predicated on common owners controlling or communicating with the firms in which they invest, though such control or communication can amplify the competitive harm.
