Only two patients with early onset Crohn's disease exhibited rare deleterious variations within NOD2: the previously described R702W variant was the sole NOD2 variant in one patient, while the second patient also carried the L1007 frameshift insertion.
Next, to rule out extraction bias as the sole source of variation observed between extraction platforms, technical replicates were compared (Figure 5, Additional file1: Table S2).
This "multiple domain feature" is better describing the IA patients' situation rather than the sole focus on variations within one domain, such as the different drugs within SUD or the different diagnoses i.e. within psychiatric disorders.
Excluding biological variation, the sole technical LC-MS measurement impression, affords median measurement variation of 11.4%.
There are, however, six transcription factor genes where non-constitutive introns are annotated as the sole source of transcript variation and for three of these, F23F12.9, zip-1 and atfs-1, the evidence is strong with many ESTs in support.
There are 24 transcription factor gene annotations with donor or acceptor splice site selection as the sole mechanism of transcript variation, but for almost all of these the alternative splice sites are just 3, 6, 9 or 12 nucleotides apart, thereby retaining the translational reading frame.
Variation partitioning analysis showed that the contribution of sole main factors to SOC variation decreased, while the interaction effect among parameters increased along the succession gradient.
Our observation that the fragile regions in the human genome are correlated with segmental duplications suggests that negative selection is unlikely to be the sole source of observed variations in fragility, assuming the segmental duplications found in the human genome are not conserved in those other species in which these regions are in fact disrupted.
The DNA and RNA reads were analyzed using the same detection procedure described above, with the sole exception that sequence variations below 0.1% (the expected sequencing error rate) were allowed in the DNA reads (as mandated by the much larger DNA coverage in this validation study).
