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Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
The sole utilization of mtDNA, typical of these recent studies, is problematic in that such data do not address the lack of independence of substitutions among nucleotide positions and ignores the potential discord between gene trees and species trees [31], [32], [33].
Moreover, the total substrate uptake rate is increased in simulations for sole utilization of acetoacetate and in combined utilization of acetate and glucose.
We found utilization of substrate combinations with a mixture of all investigated substrate more efficient compared to sole utilization of single substrates.
A well-known phenomenon among bacteria is the sole utilization of the most favored carbon source (e.g., glucose, fructose or malate) over other sugars present in the environment.
The data, summarized in Table 4, shows that sole utilization of acetoacetate is less efficient than glucose or oleate oxidation, but seems to achieve more favourable oxygen and substrate uptake rates than acetate and lactate.
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Especially sole lactate utilization required a maximal total substrate uptake rate of vs = 1.2701 mmol·min−1· l cell)−1, thus 7.84-fold higher than in case of oleate utilization.
We found that in sole oleate utilization total substrate requirement and oxygen consumption are more favourable compared to glucose, lactate and acetoacetate.
Furthermore, the oxygen and substrate demand increased to a maximum (C i + = 0, see Table 4, Figure 2B) in simulations of sole acetate utilization.
PMs for sole carbon source utilization showed that EAEC 042 can utilise 2-Deoxy-D-Ribose more effectively than E. coli MG1655 (Table S5).
Microbial abundance and composition were measured using whole-soil phospholipid fatty acid analyses and microbial activity was measured in a subset of samples using sole-carbon-source utilization analyses.
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