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To test the sole specificity of FD10 to its own antigen of FXYD6, we produced the exogenously expressed FXYD1 to FXYD5 proteins by inserting the fragments containing the FXYD1 5 cDNA coding sequences into the prokaryotic expression vector pGEX-6P-1-GST to produce the plasmids of pGEX-6P-1-GST-FXYD1 5.
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The early autoantibodies discovered by analysis of these individuals with negative samples followed by sole specificities were also among the specificities recognized by samples from individuals with multiple autoantibodies present in the first available sample.
Base sequence recognition via local changes in backbone conformation is not unprecedented but is unusual as the sole mechanism of specificity.
It is known that RBP is the sole determinant of host specificity (Dupont et al. 2004).
LTRs are often not the sole determinant of tissue specificity and their effects may therefore be hard to detect.
How such a topology is determined for different RTKs and whether it is the sole determinant of signal specificity is unclear (Kholodenko, 2007).
The adenine riboswitches have essentially the same structure except that the riboswitch cytosine is replaced with a uracil; this base pairing contact is the sole determinant of purine specificity (11− 11−.
However this is unlikely to be the sole determinant of Ser/Thr specificity, since residues containing bulky aromatic rings (Tyr, Phe or Trp) are found in the same or adjacent positions in many bacterial phosphatases.
The low acceptance of the OGTT and the non satisfying sensitivity and/or specificity of the sole HbA1c determination speak against these parameters as inclusion criteria for studies in daily practice.
In the hrHPV arm, specificity for the sole primary screening test was 92.1% for any lesion (CIN1+), 91.7% for moderate to severe lesions (CIN2+) and 91.5% for severe lesions (CIN3+).
However, the specificity and sensitivity of sole serum ferritin to aGVHD are very limited due to individual diversity.
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