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The SBA's 8(a) program provides mentoring, counseling, and access to sole-source or set aside contracts.
Arthrobacter sp. strain Rue61a is unable to grow with taurine (2-aminoethanesulfonate) as sole carbon source or as sole source of nitrogen, but taurine supports growth when supplemented as sole sulfur source (Table 2).
A Rhizopus oryzae lipase gene has been expressed in Pichia pastoris as a reporter using the formaldehyde dehydrogenase 1 promoter (PFLD1) of this organism, which has been reported to be strongly and independently induced by either methanol as sole carbon source or methylamine as sole nitrogen source.
Despite its relevance, most current models simply define ammonia uptake as the sole nitrogen source or leave amino acid uptake unbounded; only one bacterial GS-DFBA model applied kinetic parameters to define amino acid uptake, although it has not been experimentally validated [ 53].
For non-rational strain design, the recombinant cells were alternately cultured in media using xylose as the sole carbon source or SECS liquor (the leach liquor of steam-exploding corn stover) as stresses for adaptive evolution.
Therefore, non-rational, adaptive evolutions were performed in media that contained xylose as the sole carbon source or toxic lignocellulosic hydrolysates to further select for enhanced characteristics both in xylose metabolism efficiency and robustness (Heer and Sauer 2008; Kuyper et al. 2005b; Moysés et al. 2016; Sharma et al. 2016).
However, expression was repressed in total RNA samples obtained from cultures grown in the presence of VIL as sole nitrogen source, or when combined with additional nitrogen sources such as ammonium or GABA, as compared to that found in the absence of VIL (Figure 3A).
However, when media contain methylamine as sole nitrogen source or methanol as carbon source, the peroxisomal oxidases amine oxidase (AMO) and alcohol oxidase (AO) are required for growth.
Chlamydomonas is capable of growing photoautotrophically using light and CO2 as the sole carbon source, or mixotrophically (acetate and light), using acetate as a carbon source.
Utilization of crude glycerol as the sole carbon source or as a suitable co-substrate to improve the efficiency of biodigesters has also been investigated.
Pre-grown mycelia were washed and equal amounts were resuspended in 20 ml MA media containing 1%% (w/v) D-glucose or 2 mM sophorose as sole carbon source or no carbon source and were incubated for 3 h.
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