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Nonetheless, the identification of a heterozygous mutation as the sole sequence abnormality might raise doubt as to whether strong selective pressures are afforded by FHIT genomic inactivation in this tumor type.
Our sole sequence of D. podophylla was derived from Li & Lu [ 19].
The proteins with "*" were concatenated into one sole sequence before submission to WDAC.
With these parameters, the number of unique sequences (gene groups containing a sole sequence) amounted to 3747.
These two sequences cluster with strong bootstrap support, as expected, with the sole sequence from An. albimanus.
Subclade B (green) contains the annotated human and rodent Dscam-like sequences, a second sequence from both frog and dog, and the sole sequence from tetraodon.
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Each digested genome DNA is ligated with unique, short DNA sequences (barcodes adaptors) to make different plant DNAs be assembled and sequence determined together in a sole sequencing lane.
Two sole sequences (P_isotig06925 and N_isotig08536) were classified as "true" hatching enzymes belonging to the groups High Choriolytic Enzymes (HCE) and Low Choriolytic Enzymes (LCE), respectively [ 16].
Generally, these two clusters are clearly separated in all individual trees built from orthologous SNAREs, whereas Yarrowia lipolytica, as the sole sequenced member of a third cluster of the Saccharomycotina, splits earlier.
The SF1-containing clade possesses representatives from all taxa in the tree, including the sole sequences recovered from fungi, choanoflagellates (the sister taxon to metazoans), and the two nonopisthokont outgroups, Dictyostelium and Plasmodium.
The coverage of individual genomes by arCOGs ranged from 99% (strains of Sulfolobus islandicus and Methanococcus maripaludis with abundant close relatives in the set) to 73% (Nanoarchaeum equitans, the sole sequenced representative of the phylum Nanoarchaeota).
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