RSM which results from using Box Behnken design was used for further study of the influences of major factors and interaction between them on the response value, which is based on the results of sole-experiment and Plackett Burman (PB) design.
The Senegalese sole incubation experiment took place at the LEOA facility, University of Algarve, Portugal.
Indeed, the sole controlled experiment suggesting RNA-protein association appears to be the pulldown of STAT3 with biotinylated WFDC21P RNA, with specific absence of the STAT3 band in the antisense control.
Calculated rate constants (κ1) for biodegradation, or substrate disappearance, for sole substrate experiments were 1.32 mmol L−1 h−1 for benzene, 1.42 mmol L−1 h−1 for toluene, and 0.833 mmol L−1 h−1 for xylene.
It is possible that phosphorylation of all CME proteins may not be reduced in this time, but this is not the sole set of experiments on which we pin our conclusion.
All reactants were applied to the cis chamber, with the sole exception of experiments in Figures 3b and S3b (Supporting Information) where Sb2 76-93)s was alternatively applied to the trans chamber.
For experiments using xylooligosaccharides as the sole carbon source, all experiments were conducted in serum tubes using small concentrations (0.5 to 0.8 g/L) of xylobiose, xylotriose, and xylotetraose (Megazyme, Bray, Ireland) with a total volume of 12 mL per experimental replicate.
(Such mis-regulation can be sometimes eliminated during laboratory evolution experiments [ 37, 39], if growth rate maximization is the sole objective of the experiment).
To test the possibility that supplementation of one sugar might change the outcome of the culture initiated with the other sugar as the sole carbon source, the experiment was started using a culture containing 35 g/L glucose or D-xylose as the sole carbon source, with an equal amount (35 g/L) of the other carbon source being added after 24-h cultivation.
Afterwards, several experiments for sole photo-Fenton treatment were carried out in a laboratory scale photoreactor.
The sole purpose of these "experiments" is to produce data for comparison with model predictions rather than to address specific scientific hypotheses.
