The results showed that the optimized PCR solution for amplification of ITS region comprised 5 μL 10× buffer, 30 ng soil DNA template, 3.0 mmol · L−1 Mg2+, 0.2 mmol · L−1 dNTPs, 0.1 μmol · L−1 each forward and reverse primer, and 2.0 U Taq enzyme in 50 μL reaction volume.
Numerous linkages and feedback loops occur between the soil-geomorphic template, microclimate, vegetation, and animals.
The objective of this paper is to illustrate the concept of the soil-geomorphic template and its relationship to biotic change in arid and semi-arid regions.
The integral relationship between the soil-geomorphic template and biotic change is an example of how biological and geological systems are coupled and co-evolve over long-term (Quaternary landscape evolution) and short-term (human-induced desertification) time-scales.
We tested the developed amplification strategy using total DNA from a number of agricultural soils as template.
It has one Soil module, a Crop Template module which can simulate different crops by defining species input files, an interface to add individual crop models if they have the same design and interface, a Weather module, and a module for dealing with competition for light and water among the soil, plants, and atmosphere.
Extracted DNA from soil was used as template for amplification of aioA gene (~1100 bp) with primers BM1-2Fand BM3-2R as previously described (Quéméneur et al. 2008) (Additional file 1: Table S1).
We found some evidence that ants exposed to a foreign colony's soil updated their odour template and became less aggressive towards members of that colony, but this effect did not occur in all five colony pairs.
In the first PCR reaction total genomic DNA from soil sample was used as template and the primer set NVC58 and NVC65 [Coleman et al. 2006] was used, yielding a fragment with an expected of 1,100 bp.
Here, we experimentally test whether nest soil affects the odour label and template in a soil-nesting ant species, Camponotus aethiops.
