Exact(4)
We also compared the development of wild-type and acap-A−/B− cells on soil plates which more closely resemble the native environment.
We found no apparent abnormality in the morphology of tip aggregates, the early culminants or fruiting bodies from acap-A−/B− cells on soil plates.
Loss of csA did not affect development in the mutant cells under laboratory conditions, however when grown on soil plates which resembles natural conditions the mutant cells displayed reduced cell-cell adhesion, increased adhesiveness to the substratum, and slower motility, which led to their sorting out from aggregating wild-type cells [26] [26].
For soil plates, 2 g of soil was placed in a sterile Petri dish, cooled PDA medium (15 mL) was added, and soil particles were spread in the medium.
Similar(4)
In two soils, population dynamics assessed by real time PCR and the soil plate technique gave similar results.
They were isolated by direct soil plate method [ 34] and identified accordingly [ 35– 35].
KS14 was isolated from an extract of Dracaena sp. soil plated on B. multivorans C5393 [ 19].
Seed and Dennis isolated KS5 from an extract of onion soil plated on B. cenocepacia K56-2 [ 25].
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