Community genomic DNA from the anodic biofilms were extracted using FastDNA SPIN for Soil kit (MP Biomedicals, USA) and Fastprep beadbeating machine (Bio101, USA) according to the manufacturer's protocol.
DNA was extracted from 0.5 g of sediment using the MoBio DNA Power Soil Kit (MoBio Inc, Carlsbad, CA).
PCR amplification: DNA was extracted from water filters, coral, and sediment samples using the UltraClean™ Soil Kit (MoBio).
DNA from myxospores was extracted using the FastDNA Soil Kit Protocol with a Fast Prep-24 Homogenisation System equipped with QuickPrep Adapter (MP Biomedicals, Australia); the speed setting used was 6.0 for 40 s, otherwise the manufacturer's instructions were followed.
DNA was extracted from the microbial sample using either the Ultra Clean Soil DNA Kit or Power Soil Kit (MolBio, Boulder, CO).
Six ml of dense cell culture was spun down and then extracted using the FastDNA for Soil kit (MP Biomedicals, France) using the manufacturers standard protocol.
Bacterial DNA was extracted from 200 μL of the final PBS sonicate using Extrap Soil Kit Plus ver.2 (Nippon Steel Kankyo Engineering Co., Ltd., Tokyo, Japan).
Total DNA was extracted from 0.5 g of sediment using the "Power Soil" kit (MoBio Laboratories Inc., Carlsbad, CA, USA) following the manufacturer's instructions.
Total DNA was extracted from the soil fractions (rhizosphere, root-surrounding soil, and bulk soil) and root tissues using a Power Soil kit (MoBio) and DNeasy Plant kit (Qiagen), respectively, according to the manufacturer's procedure.
Genomic DNA of strain MPT was isolated from pure cultures using the Power Soil Kit (MoBio, Carlsbad, CA) according to the manufacturer's protocol and from strains CR and LT-1T as previously described (Thrash et al. 2007).
