The physicochemical characteristics of the soil assayed in the present study are shown in Table 1.
Under these conditions, the duplex soil PCR assay detected GV4 down to a concentration of 10 spores g−1 soil in non-sterile agricultural field soil.
Neither soil nor assay species taken in the present study was adapted to HQ.
We also examined the recovery of m-tyrosine in sterile and non-sterile soil without assay species when treated with 0, 4.25, 8.5, 17, 34, 68 or 136 µg m-tyrosine/g soil and incubated at 22 or 30°C.
Soil was assayed for S. enterica 24 h post-inoculation and once weekly for long term survival assays.
Finally, some insecticides and soil amendments assayed were either neutral or increased respiration.
Although bacteria do exist in these soils, the assay couldn't pick up any signs of them.
In addition to fractionated hydrocarbon analysis, culturable microbial characterisation and soil ecotoxicological assays were performed.
Thus, using state-of-the-art methods such as PCR-DGGE, micronucleus and soil enzyme assays, we aimed to investigate the ability of probiotic strain BJ1 to colonize and then degrade chlorothalonil in soil under greenhouse conditions.
