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The first was a preprogrammed software sequence that lacked a command to turn on a gyroscope necessary to reorient SOHO toward the sun in case of an emergency.
Primary sequencing data were analyzed using a sequence analysis software (Sequence Analysis 3.7; Applied Biosystems) and comparative analysis was done with the online MultAlin software (http://www.prodes.toulouse.inra.fr/multalin/ multalin.html).html
Allocation was determined by a random software sequence in ex-cell.
Following capillary electrophoresis, sequence quality was evaluated using ABI software Sequence Scanner v1.0.
Using standard base-calling software, sequence reads from bisulfite-treated EpiQuest libraries were identified.
Base calling and quality control were performed using the Real-Time Analysequenceware sequence pipeline (Illumina).
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Electropherograms of overlapping sequencing fragments were manually edited using the software package Sequence Navigator version 1.1 from PE Biosystems.
"Nobody's been able to grow it in a laboratory despite trying," said Vaughn Iverson, the computer scientist and doctoral student in oceanography at the University of Washington whose software sequenced the genome.
Primers and TaqMan probes were designed using Applied Biosystems Primer Express 3.0 software (sequences available upon request).
Sequencing was processed always in the forward and reverse directions using software Sequencing Analysis, MatchTools and Navigator (MatchTools Allele Identification package, Applied Biosystems, Foster City, CA, USA).
Using the BioNumerics v5.1 software, sequences were grouped via a pairwise clustering (pairwise alignment parameters: open gap penalty: 100%, unit gap penalty: 0%, min. match sequence: 2, max n° of gaps: 9, fast algorithm).
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