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Band intensities were quantified on the original files with the software Quantity One (Bio-Rad).
The software Quantity One Bio-Rad Laboratoriess, Hercules, CA, USA) was used for semi-quantitative densiometric analysis.
The results of the western blotting assays were quantitated by densitometric analysis (BioRad Gel Documentation System) using the analysis software Quantity 4.5.2.
The immunocomplexes signal was registered in ECL-films (Hyperfilm, GE Healthcare), and after that quantified using the software Quantity One 4.6.3 (Bio-Rad).
The expression of IL-12p40, IL-10 and iNOS and β-actin was quantified by densitometric analysis using Versa-doc Imaging system (BioRad, USA), software (Quantity one - 4.6.2 basic).
The ratio of the total plasmid DNA and the chromosomal DNA (mainly one bulky band) was obtained by measuring their band area appeared on the agarose gel by using software (Quantity One V.4.6.2); three independent measurements were performed.
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Molecular masses were determined using a commercial broad-range protein molecular mass marker and analyzed with the help of software Quantity-One (BioRad Laboratories).
Phosphor-imager output files were converted to 16-bit tiff images using the software application Quantity One (BioRad).
Software analysis determined quantity, location, and distribution of root resorption craters.
The pictures were obtained by using computer software (Bio-Rad quantity one).
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