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Cultures were pelleted in 50 ml falcon tubes by centrifugation for 15 min at 4000 rpm (Eppendorf, 5804), re-suspended in ice cold AS medium containing 10 mM MES, 10 mM MgCl2, 150 μM Acetosyringon pH 5.6 and diluted to an OD600 of 0.8 to 1 with AS medium and further incubated for 3 h at RT under soft shaking (~50 rpm).
40-um frozen sections were incubated at room temperature in soft shaking for 5 min.
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In an era of distraction, there is something beautiful about a toy that can create anything you can imagine using two simple dials and a blank screen and something important about a toy that will forgive your mistakes with a few soft shakes.
His new album, "Constant Companion," is a quiet wonder, fairly soft rock that wouldn't shake any lifelong fan of Jackson Browne or James Taylor.
To help achieve a softer dry, shake towels a lot before hanging them on the line, with a "snap".
The soft-spoken woman shaking off a monumental case of jet lag at a Manhattan apartment one recent morning was Dr. Ruth Kiew, 56, the keeper of the herbarium and library of the Singapore Botanic Gardens, and one of the world's great experts on tropical begonias.
The enemy's strategy, the Birchers warned, was to aim at the soft underbelly and shake it like a bowlful of jelly.
We had several sightings of them, and when they took to the air the beating of their wings sounded like soft blankets being shaken out.
A concussion is an injury to the brain caused by a sudden impact to, or spin of the head during which the brain (a soft organ) is shaken so hard that the buffering cerebral spinal fluid surrounding it can't prevent it from banging against the hard skull.
The free kick comes to nothing beyond a hefty blow to the head for Algeria keeper Chaouchi who rolls around like a big soft boy before getting up shaking his head bravely like he's been chinned by Mr T and somehow survived.
This was followed by shaking, pounding and "rearrangement" of soft tissue.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com