This step is followed by soft baking for 5 min at 100°C and then rapid thermal processing (RTP) annealing for 1 min in an O2 environment at various annealing temperatures ranging from 450°C to 700°C.
Table 4 Ratios between bone and soft tissue after 5 min p.i. of 177Lu-complexes in Wistar rats Bone/organ [177Lu]citrate [177Lu]NO2APBP177Lu]BPAPD [177Lu]DO2A PBP 2Lu]NO2APBP [177Lu]DOTA MBP 2 [177Lu]DOTA(MBloodBlood n.d.
After soft bake for 3 min at 95 °C, the layer was exposed by ultraviolet (UV) of 365 nm wavelength for 6.4 s (160 mW) using MA-6 Aligner (Karl-suss, Germany).
Sautee for 10 min until soft, then add chiles and cook for 3 minutes.
40-um frozen sections were incubated at room temperature in soft shaking for 5 min.
Following overnight storage at −20°C, cells were then centrifuged at 1000 × g for 5 min using soft acceleration.
Each pellet was washed in 500 μl dPBS and suspension was centrifuged at 1000 × g for 5 min using soft acceleration, after which supernatant was removed.
For artificial contamination, hands were washed for one min with soft soap, dried with paper towels, immersed in the contamination fluid up to the mid-metacarpals for 5 s with fingers spread, and allowed to dry for 3 min [ 6].
Cell suspensions were combined with the medium in the appropriate 15-ml tubes, and cell pellets were collected by centrifugation at 1000 × g at 4 °C for 5 min using soft acceleration.
For artificial contamination, hands were washed for one min with soft soap, dried with paper towels, immersed in the Escherichia coli contamination fluid up to the mid-metacarpals for 5 s with fingers spread, and allowed to dry for 3 min [ 11].
