The selected areas were brushed with a manual (Oral-B soft indicator Regular 40; Oral-B laboratories, Belmont, CA, USA), electric rotating (Oral-B Professional Care 7850 DLX; Braun GmbH, Kronberg, Germany), or sonic (Oral-B Sonic Complete; Braun GmbH, Kronberg, Germany) brush for 20 s, with the brush attached to a home-made moving tray, involving 20 strokes back and forth.
Even if the real economy only lives up to half the expectations recently created by soft indicators, any fears of a technical recession should turn out to have been unjustified.
The project will enter the data produced by different institutions and collected by the BiH Ministry of Human Rights and Refugees, along with qualitative data collected by NGOs at community level into a unique system, enabling cross-referencing administrative data with case studies and soft indicators collected by NGOs.
So do stubbornly soft economic indicators and stock and bond prices that are expensive by historical standards.
When overexpressed in a leukemia cell line (K562), BP1 increased the ability of cells to grow in soft agar, an indicator of leukemogenic potential, up to 45-fold [ 11].
Use of temporary external fixation is also likely an indicator of soft tissue injury or soft tissue problems, rather than an independent risk factor on the basis of colonized pin tracks or other factors, an argument supported by the fact that temporary external fixation was not included in the best multivariable model.
The bottom agar layer stabbed by the test strains was subsequently overlaid with a thin layer of a soft agar containing the indicator strain.
When necessary, the bottom agar layer supporting the growth of the test strains was kept in chloroform vapors for 15 min and then overlaid with a soft agar containing the indicator strain.
After filter sterilization of relevant compounds, approximately 100 μl was filled to the wells which had been prepared previously by overlaying LB soft agar including the indicator strains Micrococcus luteus NCBI8166 and Escherichia coliATCC25922 on to the Müller-Hilton agar plates, then 5 mm wells were created with cork borer respectively.
"Homesick" was successful on both Christian and mainstream radio, peaking at the top on the Radio & Records Christian AC Indicator and Soft AC/INSPO charts, number 3 on the Billboard Hot Christian Songs and Hot Christian AC charts and the Radio & Records Christian AC chart, as well as peaking at number 9 on the Billboard Adult Contemporary chart.
We then tested colony formation in soft agar as an indicator of cellular transformation.
