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Finally 200 litres of media (Bioreactor BR600, Belach Bioteknik AB, Stockholm, Sweden) was inoculated and kept under soft agitation (20 rpm) to be able to maintain a constant temperature of 30°C.
After 1 h incubation at room temperature with soft agitation, the beads were collected by centrifugation and the supernatant removed (this supernatant is designated the "unbound fraction").
Ovaries were collected, separated and incubated in medium containing 5 μM Lysotracker (DND-99, Molecular Probes) in soft agitation for five minutes at room temperature in the dark.
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SEDDS can be orally administered in soft or hard gelatin capsules and form fine relatively stable oil−in−water (o/w) emulsions upon aqueous dilution owing to the gentle agitation of the gastrointestinal fluids.
Her agitation made me agitated.
When the solution cools down, it further thickens and forms a soft gel that easily breaks down under agitation and mixing.
If the manufacturer's instructions allows it, a bath is a good place as you can submerge it in water with the appropriate cleaning solution recommended on the label, or very soft soap such as Ivory Flakes, without unnecessary agitation.
Poulenc's affecting score is soft-spoken and restrained, even in moments of agitation and violence.
Usually, she is measured, soft-spoken, but there was the slightest tone of agitation in her voice: "It's important to remember that this is Lent, and meditations on the Passion of Christ are an important part of the cultural interpretation of human suffering.
Everyone inside was costumed, to some degree, but in his agitation Stephen imagined that it was actually he, in his soft windowpane jacket and pressed shirt and woollen pants — he and not the dead and undead thronging about him, blocking his way — who was wearing a costume.
An easy way to clean your dirty shower curtain is to run it through the washing machine with small and soft items like hand towels, wash cloths, socks, etc. Use cool water and plenty of soap and agitation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com