After purification via chromatography, samples were stored in 10 mM sodium phosphate buffer.
The column was previously equilibrated with 20 mM sodium phosphate buffer, pH 5.7.
pH 7 sodium phosphate buffer in a 20 ml vial with a small stirbar.
Purified proteins were stored at −20 °C in 10 mM sodium phosphate buffer pH 7.4.
Proteins were dialysed into 100 mM sodium phosphate buffer pH 7.0 and protein concentration was adjusted to 0.1 mg/mL.
Pellets were rinsed with 1 M NaCl and resuspended in 50 mM sodium phosphate buffer (pH 7.5).
Aliquots were diluted 1 20 with 50% sodium phosphate buffer (50 mM, pH 7.5, containing 50% methanol).
PD-10 desalting columns (GE Healthcare) were used for buffer exchange (50 mM sodium phosphate buffer, pH 5.7).
The dissolution media were sodium phosphate buffer, pH 7.0, and ammonium acetate buffer, pH 6.8.
The pellet was resuspended in 100 mM sodium phosphate buffer, pH 7.0 and the solution clarified by centrifugation at 27,000×g for 20 min.
Microspheres were washed in 10 mM sodium phosphate buffer, pH 7.4 supplemented with 0.1 mg ml−1 bovine serum albumin (BSA) and 0.05% Tween-20.
