Sentence examples for sodium chloride buffer from inspiring English sources

Briefly, 50 ml of buffy coat were diluted (2∶3, v∶v) in sodium chloride buffer (0.9%, w∶v).

MHC-peptide mixtures was incubated for 3 days at 37°C in 100 mM sodium citrate (pH 5)/50 mM sodium chloride buffer (pH 5.5), containing 0.25% octylglucoside, 185 ug/ml iodoacetamide (IAA), 25 nM EDTA, 0.1% NaN3 and freshly added 0.5 ug/ml PMSF.

Beads were washed in the presence of either a 150 mM ammonium acetate buffer ('low salt') or a 2 M sodium chloride buffer ('high salt').

Samples were loaded and washed in 0.5 mL 50 mM Tris, pH 7.4, 0.5 M sodium chloride buffer and retained components were eluted using 0.5 mL 50 mM glycine pH 3.0 buffer.

Peptides were chromatographed using a borate buffer system, namely 10 mM sodium borate, pH 9.3 (Buffer A) and 10 mM sodium borate, pH 9.3 + 0.5 M sodium chloride (Buffer B) and eluted using an exponential elution gradient into 12 × 750 μl fractions.

Frozen cells were thawed on ice and resuspended in 0.1 M sodium phosphate buffer pH 7 with 20 mM imidazole and 0.5 M sodium chloride (buffer A) containing 1 mg/mL lysozyme and protease inhibitor mix (Roche Complete Protease Inhibitor Mix, EDTA-free) and re-frozen at -80°C.

The M. hyopneumoniae antigen was prepared as described [ 22]; M. hyopneumoniae at passage 15 was harvested by continuous flow centrifugation at 70,000 x g and resuspended in Tris-sodium chloride buffer.

The placebo consisted of the viral formulation buffer, composed of sodium chloride, Tris buffer, trehalose (low endotoxin), magnesium chloride, monooleate (Tween 80) and water for injection.

Several factors significantly change the adsorption capacity of both Alhydrogel and Adjuphos, including molecular weight of protein antigens, sodium chloride, phosphate buffer, denaturing agents, and size of aluminum particles.

For endophyte extraction, sterile leaves were crushed in autoclaved mortars with 0.85% sodium chloride as buffer.

The gels were then transferred into 0.1 M sodium formate, pH 3.5 containing 0.15 M sodium chloride (hyaluronidase buffer).