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PIIa divides to provide a posterior socket cell and an anterior hair cell.
As shown in Fig 2B frame 12, the lower cluster contains outer cells (as shown by the presence of a Su(H) positive socket cell, yellow coloured cell).
Among the final four cells present in this cluster one was a Su(H) positive socket cell (yellow cell) and one a neuron (blue cell, the inset shows a z-reconstitution to illustrate the nucleus that is not situated in the same plane as the other cells).
Socket cell DA also promotes the refractory period.
Socket cell DA and neuronal ACh regulate ejaculation and the refractory period.
Thus, we analyzed the contribution of the neurotransmitter DA in socket cell function during mating behavior.
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Each division from the SOP is therefore asymmetric and generates non-neuronal cells such as posterior socket cells or anterior hair cells [15] [20].
We identified socket cells by the characteristic strong accumulation of Su(H) and glial cells by the expression of Repo [14], [18].
The division of pIIa leads to the formation of the outer cells of the organ (the shaft and socket cells) [13], [18] (Fig. 1A).
This was confirmed by immuno-detection (frame 9) showing that the left cluster was formed by four outer cells: two Su(H) positive socket cells (yellow cells) and two shaft cells (green sibling cells), showing that the organ originated from two pIIa cells.
Sp = spicule, So = socket cells.
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