We present the X-ray structure at 1.55 Å resolution of exo-inulinase from Aspergillus awamori, a member of glycoside hydrolase family 32, solved by single isomorphous replacement with the anomalous scattering method using the heavy-atom sites derived from a quick cryo-soaking technique.
The researchers adopted three different techniques for soaking up energy from phonons.
The ground samples were extracted using the maceration technique by soaking the samples in the solvents for 24 h at room temperature, followed by filtration using Whatman No. 1 filter paper.
Other tissues were harvested using standard techniques, soaked or perfused with 1% formaldehyde, and homogenized in neutralization buffer followed by ChIP.
In this work, we determined the X-ray crystal structures (at 1.54 and 1.76 Å resolution) of HbHNL complexes with two chiral substrates – mandelonitrile and 2,3-dimethyl-2-hydroxy-butyronitrile – by soaking and rapid freeze quenching techniques.
Needing a break, he returned to South America in 2005 and traveled for two years, soaking up the vibrant flavors and techniques of culinary cultures that know how to make great food with humble ingredients.
The study includes fabrication of bucky papers, resin impregnation by different techniques, i.e., soaking, hot-compression and vacuum filtration, followed by β-stage curing and the integration with carbon fiber prepregs to produce CFRP composites with bucky paper interleaves.
Acute wound cleansing incorporates three different techniques: compresses, irrigation, and soaking (Table 3).
Processors treat tissues with a variety of so-called secondary sterilization techniques -- gamma radiation, disinfectants and soaking in antibiotics.
It tames raw onions with a Turkish technique described as "killing" the onion: soaking it in salted water to draw out some of its harshness.
