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About 10 g of the grounded leaves of P. europaea and P. auriculata plants were soaked separately in 1 l of three different solvents (water, methanol, and acetone), and each of the extracts was placed in a shaker device at 100 rounds per minute for 72 h at room temperature and then stored in a refrigerator for 4 days.
J2s of H. schachtii and G. pallida were soaked separately in 20 µl of the dye solution in the dark at room temperature for 16 24 h.
Briefly, three 100 g portions of the dried powdered plant were soaked separately in 500 ml of distilled water, ethanol (98%%) and η-hexane (99 %), for 72 h.
20 μl of each extract was soaked separately into sterile discs (Hi Media, Mumbai, India), and the discs were dried in oven for 4 hours at 35°C.
Briefly, 100 g of the powdered plant material was weighed out and soaked separately in 1000 ml of solvents for 72 hours.
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Launder your used wipes with cloth diapers, or soak separately in a container of water, vinegar and baking soda mixture before washing.
Powdered material of each plant was soaked in methanol separately at room temperature.
DNA probe immobilisation duration was determined by separately soaking nine units of AcMP-AuNP-modified SPEs in 300 μL of 5 μM arowana DNA probe solution for 1, 2, 3, 5, 6, 7, 8, 12, and 18 h, before reaction with 5 μM of cDNA in DNA hybridisation buffer (0.05 M of Na-phosphate buffer at pH 7.0) containing 1 mM of antraquinone redox intercalator and 2 M of NaCl.
Replace your vehicle's hub caps (which you may want to soak and clean separately) to finish this job.
Two hundred-gram powdered leaves of A. indica and M. azedarach were soaked in 2 L methanol separately for 12 days.
Wash severely stained or oily clothes separately, one oil soaked shirt can make 20 brown socks.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com