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We used six fish-versatile long PCR primers in the following four combinations (S-LA-16S-L + S-LA-16S-H; L2508-16S + H1065-12S; L2508-16S + H12293-Leu; L12321-Leu + S-LA-16S-H; for locations and sequences of these primers, see Miya & Nishida [ 17, 38], Inoue et al. [ 39, 40], Ishiguro et al. [ 41], Kawaguchi et al. [ 42]) so as to amplify the entire mitogenome in two reactions.
In 1912 de Forest conceived the idea of "cascading" a series of Audion tubes so as to amplify high-frequency radio signals far beyond what could be accomplished by merely increasing the voltage on a single tube.
To detect the genome and/or antigenome, two primers were chosen so as to amplify a 221 nt fragment (L-Tr) encompassing L gene end and trailer.
The other part was used to hybridize with USA probe so as to amplify the ECL signal and thus improve the sensitivity of the assay.
Such stereotypical representations, both in images and in words, are not due to a lack of Palestinian voices, as they exist in great abundance, but the media's negligence in communicating with these voices and giving them a platform so as to amplify them.
All primer pairs were designed using the Primer3 software V.0.4.0 [ 28, 29] so as to amplify stretches between 600 and 1.300 bp and cover a part of the promoter and the first exons and introns [ 28, 29].
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Primers for EGR2, MECP2 and GAPDH were designed using primer3 software with one of the primers of each primer pair spanning an intron exon junction so as not to amplify genomic DNA (Supplementary Material, Table S3).
A convex function is so needed to amplify.
Savor positive experiences; doing so tends to amplify the pleasant emotions that you might be feeling.
So, when we feel we have done so, we want to amplify and extend that feeling.
He warned that failure to do so is certain to amplify the terror threat at home.
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