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We examined the subcellular localization of SNB-1 (synaptobrevin) using a transgene, juIs1[Punc-25::SNB-1::GFP], SNB-1 GFP]ses a SNB-1::GFP chimeric prothat in thexpressesic motor neurons [66].
To test this possibility, we examined the localization of SNB-1 using odIs1, a transgene that expresses a SNB-1 GFP SNB-1 GFPsion in the GLR-1-exproteing interneurons ofusioncentral nervous system.
To determine whether our newly identified rpm-1 mutations also impair presynaptic bouton formation, we examined the subcellular localization of SNB-1 (synaptobrevin) using juIs1, a transgene that expresses a SNB-1 GFP SNB-1 GFPsion in motorneurons.
The number of SNB-1 GFP SNB-1 GFP greatly reduced, and the SNB-1::GFpunctata exhibited greater variability in size and spacing than synaptic GFP-puncta of wasd-type animals (Figreatly & D).
Loss of SNB-1 GFP SNB-1 GFPost severe appearedistal tips of the dorsal D neurons, although irregular size SNB-1::GFP puncta occurred all along the dorsal cord.
snb-1 codes for synaptobrevin, a transmembrane protein that by immunohistochemistry has been shown to localize to synaptic vesicles [24].
Heterozygous ju89 animals display locomotion and SNB-1-GFP phenotypes intermediate between wild-type and ju89 homozygotes.
snb-1 encodes a neuronal v-SNARE synaptobrevin (VAMP) which is localized primarily to synaptic vesicles in C. elegans [12].
To test these possibilities, we examined the presynaptic marker synaptobrevin using a synaptobrevin::GFP (SNB-1 GFP) SNB-1 GFP].
In wild-type animals, SNB-1 GFP SNB-1 GFPed to small (∼0.5 misron) boutons alocalizedventoal cord (Fig. 3B).
The majority of abnormal SNB-1 puncta were smaller than wild-type puncta, but unusually large GFP puncta were sometimes also observed along the dorsal cord.
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