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This was further confirmed by the results obtained for the 12 reference sequenced contigs (17.0 Mb for the WGP map vs. 17.2 Mb for the SNaPshot map).
The distribution of the contig size showed that in fact, the SNaPshot map contains many more small contigs (23.6% of contigs < 200 kb) than the WGP map (12.4% of contigs < 200 kb; Additional file 1).
We used a subset of 27% of the wheat 3B chromosome BAC library [ 23] representing 230 Mb to establish a WGP physical map and compare it with a SNaPshot map constructed using the same BACs.
Interestingly, the higher number of singletons did not affect greatly the size of the WGP assembly and the length of the WGP map (199 Mb ± 42) did not significantly differ from the length of the SNaPshot map (236 Mb ± 65).
Then, the results showed that the WGP map covers the same length than the SNaPshot map but with 30% less contigs and, more importantly with 3.5 times less mis-assembled BACs.
The first BAC contig sequencing results obtained on chromosome 3B [ 28] using the physical map established with the SNaPshot technology [ 23] suggested that about 10% of the BACs were mis-assembled (unpublished data) and this was confirmed in our study (estimated mis-assembled BACs in the SNaPshot map: 9.5%).
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A total of 174 F2 plants was used for RFLP and SNaPshot mapping and 560 F2 were used for Illumina GoldenGate assays.
Here we report the first genome-wide, BAC-based SNaPshot physical map of the Brassica "A" genome.
To increase the stringency of the analysis, we performed a second tag filtering with the parameters used previously for assembling the 3B SNaPshot physical map [ 23].
Where Larkin offers an evocative snapshot, Sprackland maps every inch of the lost domain.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com