Sentence examples for snap-cooled from inspiring English sources

Solutions of SL1067 were heated to 95 °C for 10 min and then snap-cooled on ice.

2'F-Py RNAs (800-300 pmol) were heated at 85°C for 5 min in 1.5 ml of DMEM serum free, snap-cooled on ice for 2 min, and allowed to warm up to 37°C.

To synthesize cDNAs, 2 µl Oligo dT primer (50 µM), 2 µl dNTP mixture (10 mM) and 5 µl RNase-free H2O were added to each tube, and the tubes were heated at 65°C for 5 minutes, then snap-cooled on ice immediately.

Precipitated RNAs were re-dissolved in water, mixed with 500 pmol of the 5' end RNA adapter, A3 (5'-GAU AUG CGC GAA UUC CUG UAG AAC GAA CAC UAG AAG AAA: Dharmacon Research), heat-denatured at 95°C for 5 min, then snap-cooled on ice.

The precipitant was diluted by adding 50 µL sterile ddH2O, boiled for 5 min, snap-cooled on ice for 3 min, extracted by phenol/chloroform (25∶24), and the supernatant was used as the template for PCR to obtain the ssDNA pool for the next round of selection.

DNA samples were denatured at 99°C for 5 minutes then snap-cooled in iced water.

In details, 5 μl of individual amplicons were mixed with 10 μl of loading buffer (75% formamide, 2.5 mM Tris, 0.0075 bromophenol blue, 0.0075% xylene cyanol, 15% glycerol, 15 mM EDTA), denaturated at 96 °C for 15 min and snapped cooling on a freeze block (− 20 °C) for 5 min.

The Z2's waterproof and dustproof special powers just feed into that function — allowing the user to be unafraid to take the Z2 out in a variety of environments to film and snap cool stuff.

The pools were denatured at 95°C, snap cooled and used to perform the second round of selection using the same procedure as described for first selection.

10 µl of the purified RNA sample (above) was incubated at 95°C for 3 minutes and snap cooled on ice for 1 minute.

The plate was then denatured at 95°C for 3 minutes and snap cooled on ice for 5 minutes and placed in ABI Prism 3100 genetic analyzer for electrophoresis.