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The SMS treatment was made 1 time.
For SMS treatment of entire leaves, the SMS treatment was carried out on both sides of the main vein.
SMS treatment leaves were incubated in covered trays at high humidity (referred to as humid conditions); in some cases the trays were left uncovered after SMS treatment (referred to as dry conditions) at the same laboratory conditions.
For the time course experiment the SMS treatment was repeated 1, 5, 7 and 10 successive times, for the others experiments the SMS treatment was repeated 10 successive times.
Inoculation with B. cinerea was performed within 10 min after SMS treatment, by placing a droplet of spores on the SMS-treated site.
The expression of so-called touch genes previously associated with mechanical stimulation such as TCH3, TCH4, CML24 and CML39[ 17] was also induced 0.5 to 1 h after SMS treatment.
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In period 2 of both years, OM digestibility was greatest for the SM treatment (P < 0.001).
Heifer average daily gain tended to be lower on the TMR-SM treatment compared with the TMR-L and TMR-SC treatments (0.78 vs. 0.94 kg/d).
The milk yield and 4% fat-corrected milk yield of cows in the SM treatment tended to increase compared with those in the S and M treatments.
Recovery of L3 was maximal (28%) with a SM treatment of 30% and simulated rainfall on day 3. Faecal moisture was significantly affected by collection day, SM treatment, rainfall amount and rainfall timing with significant interaction between many of these effects (P < 0.05).
Cows fed SM also tended to have higher NDF and ADF apparent digestibility values than did cows fed S or M. Plasma Cu concentration significantly increased for the SM treatment compared with the S and M treatments.
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