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These flow rates represent cigarette smoke concentrations of 7-100%.
Ames (YG1042) reverse mutation data correlated with increased smoke concentrations.
A clear cytotoxic dose response was observed with increased smoke concentrations (12.0, 8.0, 4.0 and 1.0 L/min).
We aimed to quantify how future scenarios of land management could affect local and regional exposure to smoke concentrations.
For example, using the Borgwaldt RM20S exposure system we observed a deposition range between 25.75-0.22 μg/cm2 for smoke concentrations tested [22].
The system is highly versatile because it allows simultaneous exposure of up to six replica inserts while applying multiple dilution airflows (i.e., testing seven different smoke concentrations).
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The pertinent parameters such as dust concentration, smoke density, oxygen concentration and air temperature have been simulated.
Pre-chamber losses were calculated by the difference in smoke concentration between the point of smoke generation (100%) and the chamber, and chamber deposition was calculated by the difference in smoke concentration before and after the chamber.
We found that the effects using a cell viability assay were correlated with the smoke concentration for both cell lines.
Spectrofluorometric quantification of particle deposition within chambers indicated a positive correlation between smoke concentration and particle deposition.
For each smoke concentration, three samples were analyzed and a total of three independent exposure runs were conducted.
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