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As expected, the smear patterns became more obvious as the duration of boiling increased.
Our finding is similar to the smear patterns reported by many investigators for both fibroin and sericin after being processed in aqueous media at elevated temperatures.
The analysis showed (Fig. 2) typical smear patterns for BMSF, APSF and their blend, such indicating that the original polypeptides were degraded during degumming due to hydrolytic reactions at the elevated temperatures and pH values employed in the process.
Lanes 1 and 2 showed smear patterns for group III, lanes 3 and 4 showed lack of DNA laddering for group I, lane 5 marked DNA ladder in group II, and lanes 6 and 7 showed lack of DNA laddering for group IV.
Lastly, quantification of smear patterns in autoradiograms is a quite error-prone method.
Collagen was significantly degraded by incubation with the lysosomal fraction from TGF- β1-treated BI-1 cells, as revealed by smear patterns in the gels.
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The same sericin, when extracted in autoclave, showed an undefined smear pattern.
Staining revealed a smear pattern (Figure 1), which strongly suggests that the polypeptides in sericin were degraded during processing by autoclaving owing to hydrolytic reactions.
A contiguous smear pattern covering a large array of molecular masses (between 10 and 250 kDa) indicated significant hydrothermal degradation resulting in a large number of polypeptides, while proteins with higher molecular mass are still present.
Sericin collected directly from the cocoons (Teramoto et al. 2005) or glands (Teramoto et al. 2006) of the Sericin Hope silkworms exhibited an electrophoretic pattern comprising at least five distinct polypeptides; however, these bands could no longer be identified following autoclaving of the samples as they merged into a smear pattern practically identical to that shown in Figure 1.
Sericin isolated from a fibroin-deficient B. mori mutant strain (185 Nd-s) by extraction at 35 °C in lithium bromide solution displayed four distinct bands between 60 and ~300 kDa, while the product extracted by alkaline boiling showed a smear pattern from ~10 to ~250 kDa (Wang et al. 2014).
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